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North Harbour (Sydney) Aquatic Reserve is located between North Head and Dobroyd Head in the northern part of Sydney Harbour, covering an area of approximately 260 hectares.

Historically, the reserve was the site of some of the first marine specimen collecting conducted in the 1830s by the superintendent of the Quarantine Station.

The aquatic reserve includes a variety of habitats, including rocky shores, sandy beaches, nearshore reefs, sandy seabed and harbour waters up to around 20 m deep.

Other than finfish, no marine animals or plants can be harmed in the Reserve.